Human pancreatic microenvironment promotes β-cell differentiation via non-canonical WNT5A/JNK and BMP signaling

DOI: 10.1038/s41467-022-29646-1

Authors: Jolanta Chmielowiec; Wojciech J. Szlachcic; Diane Yang; Marissa A. Scavuzzo; Katrina Wamble; Alejandro Sarrion-Perdigones; Omaima M. Sabek; Koen J. T. Venken; Malgorzata Borowiak

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “E8” for “1” days. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “ESC” should be obtained at the end of culture.