Transplantation of hPSC-derived pericyte-like cells promotes functional recovery in ischemic stroke mice

DOI: 10.1038/s41467-020-19042-y

Authors: Jiaqi Sun; Yinong Huang; Jin Gong; Jiancheng Wang; Yubao Fan; Jianye Cai; Yi Wang; Yuan Qiu; Yili Wei; Chuanfeng Xiong; Jierui Chen; Bin Wang; Yuanchen Ma; Lihua Huang; Xiaoyong Chen; Shuwei Zheng; Weijun Huang; Qiong Ke; Tao Wang; Xiaoping Li; Wei Zhang; Andy Peng Xiang; Weiqiang Li

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human cell in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “1” days.

Step 2: mesoderm 1

day 1 ~ 6

culture “adherent” human cell in “MIM” for “5” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “5” days.

Ideally, “MP” should be obtained at the end of culture.