Extracellular vesicles from human embryonic stem cell-derived cardiovascular progenitor cells promote cardiac infarct healing through reducing cardiomyocyte death and promoting angiogenesis

DOI: 10.1038/s41419-020-2508-y

Authors: Qiang Wu; Jinxi Wang; Wilson Lek Wen Tan; Yun Jiang; Shihui Wang; Qiang Li; Xiujian Yu; Jiliang Tan; Shenyan Liu; Peng Zhang; Zenia Tiang; Zhongyan Chen; Roger Sik-Yin Foo; Huang-Tian Yang

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “ESC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 4

culture “adherent” human ESC in “DMEM/F12” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “CVPC” should express “SSEA1”, “MESP1”, “MEF2C”, “GATA4”, “ISL1”, “NKX2-5” at the end of culture.