Graft immaturity and safety concerns in transplanted human kidney organoids

DOI: 10.1038/s12276-019-0336-x

Authors: Sun Ah Nam; Eunjeong Seo; Jin Won Kim; Hyung Wook Kim; Hong Lim Kim; Kyuryung Kim; Tae-Min Kim; Ji Hyeon Ju; Ivan G. Gomez; Kohei Uchimura; Benjamin D. Humphreys; Chul Woo Yang; Jae Yeon Lee; Jin Kim; Dong Woo Cho; Benjamin S. Freedman; Yong Kyun Kim

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “GelTrex” at “3” “%”. Medium should contain factors shown in the following table.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “RPMI”. For ECM, use “GelTrex” at “3” “%”. Medium should contain factors shown in the following table.

Step 3: mesoderm 1

day 2 ~ 4

culture “adherent” human PSC in “RPMI” for “2” days. For ECM, use “GelTrex” at “3” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.