A Role for the Long Noncoding RNA SENCR in Commitment and Function of Endothelial Cells
DOI: 10.1038/mt.2016.41
Authors: Mounia Boulberdaa; Elizabeth Scott; Margaret Ballantyne; Raquel Garcia; Betty Descamps; Gianni D Angelini; Mairi Brittan; Amanda Hunter; Martin McBride; John McClure; Joseph M Miano; Costanza Emanueli; Nicholas L Mills; Joanne C Mountford; Andrew H Baker
Step 1: mesoderm 1
day 0 ~ 3
culture “spheroid” human ESC in “unspecified medium” for “3” days. Medium should be changed once in “3” days.
Ideally, “mesoderm” should express “BRACHYURY”, “MIXL1”, “CD326”, “CD56”, “NANOG”, “POU5F1” at the end of culture.
Step 2: endothelial 1
day 3 ~ 7
culture “adherent” human ESC in “EGM2” for “4” days. Medium should contain factors shown in the following table, and should be changed once in “4” days.
| concentration | unit | |
|---|---|---|
| FBS | — | — |
Ideally, “EC” should express “CDH5”, “PECAM1”, “TRA1-60”, “CD43”, “SENCR”, “FLI1”, “ETV2”, “MALAT1” at the end of culture.