Transient secretion of VEGF protein from transplanted hiPSC-CMs enhances engraftment and improves rat heart function post MI

DOI: 10.1016/j.ymthe.2022.08.012

Authors: Xuefeng Ai; Bingqian Yan; Nevin Witman; Yiqi Gong; Li Yang; Yao Tan; Ying Chen; Minglu Liu; Tingting Lu; Runjiao Luo; Huijing Wang; Kenneth R. Chien; Wei Wang; Wei Fu

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” unknown species cell and human PSC in “E8” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “PSC” should express “SOX2”, “POU5F1”, “NANOG”, “Tra-1-60” at the end of culture.