cAMP/EPAC Signaling Enables ETV2 to Induce Endothelial Cells with High Angiogenesis Potential

DOI: 10.1016/j.ymthe.2019.11.019

Authors: Jae-Jun Kim; Da-Hyun Kim; Jin Young Lee; Byung-Chul Lee; Insung Kang; Myung Geun Kook; Dasom Kong; Soon Won Choi; Heung-Myong Woo; Dong-Ik Kim; Kyung-Sun Kang

Step 1: endothelial 1

day 0 ~ 2

culture “adherent” human Fibroblast in “EGM2” for “2” days. “Fibroblast” should be “upregulated” for “ETV2”. For ECM, use “Collagen” at “0.02” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “EC” should express “PECAM1”, “CDH5”, “VWF”, “KDR”, “CD34”, “TIE2”, “CLDN5”, “EFNB2”, “HEY1”, “NOTCH4” at the end of culture.

Step 2: endothelial 2

day 2 ~ 4

culture “adherent” human EC in “EGM2” for “2” days. “EC” should be “upregulated” for “ETV2”. For ECM, use “Collagen” at “0.02” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “EC” should express “PECAM1”, “CDH5”, “VWF”, “KDR”, “NOTCH4”, “HEY1”, “SOX18”, “FOXO1”, “TSPAN12”, “EDN1”, “TIE2”, “CD34”, “CLDN5” at the end of culture.