The growth of endothelial-like cells in zebrafish embryoid body culture

DOI: 10.1016/j.yexcr.2020.112032

Authors: Muhammad Ibrahim; Bing Xie; Michael K. Richardson

Step 1: endothelial 1

day 0 ~ 4

culture “spheroid” zebrafish cell in “LDF medium” for “4” days. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “EC” should express “fli1”, “kdrl” at the end of culture.

Step 2: endothelial 2

day 4 ~ 8

culture “adherent” zebrafish cell in “LDF medium” for “4” days. For ECM, use “collagen type-I + Geltrex” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “EC” should express “fli1”, “kdrl”, “vegfab”, “vegfaa”, “notch1a”, “notch1b” at the end of culture.