A protocol for rapid pericyte differentiation of human induced pluripotent stem cells

DOI: 10.1016/j.xpro.2020.100261

Authors: Elizabeth A. Aisenbrey; Elizabeth Torr; Hunter Johnson; Cheryl Soref; William Daly; William L. Murphy

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “E8” for “2” days. For ECM, use “Matrigel” at “0.06” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 2 ~ 4

culture “adherent” human PSC in “E8” for “2” days. For ECM, use “Matrigel” at “0.06” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 3: endothelial 1

day 4 ~ 8

culture “adherent” human PSC in “DF3S medium” for “4” days. For ECM, use “Matrigel” at “0.06” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “EC” should express “CD34”, “PECAM1” at the end of culture.