Generation of functional endothelial cells with progenitor-like features from murine induced pluripotent stem cells

DOI: 10.1016/j.vph.2016.07.008

Authors: Neli Kachamakova-Trojanowska; Witold Nowak; Krzysztof Szade; Jacek Stepniewski; Karolina Bukowska-Strakova; Monika Zukowska; Hevidar Taha; Antonina Chmura-Skirlinska; Michael Beilharz; Jozef Dulak; Alicja Jozkowicz

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” mouse PSC in “unspecified medium”.

Ideally, “PSC” should express “SSEA-1”, “NANOG”, “PECAM1”, “CD34”, “Tie-2”, “CD105” at the end of culture.

Step 2: endothelial 1

day 1 ~ 5

culture “adherent” mouse PSC in “alpha-MEM” for “4” days. For ECM, use “Collagen” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Step 3: endothelial 2

day 5 ~ 12

culture “adherent” mouse PSC in “alpha-MEM” for “7” days. For ECM, use “FN1” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “7” days.

Ideally, “EC” should express “CD34”, “Tie-2”, “Sca-1”, “CD102”, “CD106”, “VWF”, “FLT1”, “KDR”, “E-selectin”, “CDH5”, “NOS3”, “c-kit”, “MCAM”, “miR-21” at the end of culture.