Evaluation of the impact of iPSC differentiation protocols on transcriptomic signatures
DOI: 10.1016/j.tiv.2024.105826
Authors: Vidya Chandrasekaran; Sara Wellens; Aurore Bourguignon; Ivo Djidrovski; Leonie Fransen; Sreya Ghosh; Zahra Mazidi; Cormac Murphy; Carolina Nunes; Pranika Singh; Melinda Zana; Lyle Armstrong; András Dinnyés; Johannes Grillari; Regina Grillari-Voglauer; Martin O. Leonard; Catherine Verfaillie; Anja Wilmes; Marie-Gabrielle Zurich; Thomas Exner; Paul Jennings; Maxime Culot
Step 1: iPSC/ESC maintainance
day 0 ~ 1 (duration missing in database; assumed 1 day)
culture “adherent” human PSC in “mTeSR”. For ECM, use “Geltrex” at “N/A” “N/A”. Medium should contain factors shown in the following table.
| concentration | unit | |
|---|---|---|
| EDTA | 0.02 | % |
Ideally, “PSC” should be obtained at the end of culture.
Step 2: endothelial 1
day 1 ~ 5
culture “spheroid” human PSC in “RPMI medium” for “4” days. Medium should contain factors shown in the following table, and should be changed once in “4” days.
| concentration | unit | |
|---|---|---|
| CHIR90021 | — | — |
| purmorphamine | — | — |
| BMP4 | — | — |
| SB431542 | — | — |
| VEGF | — | — |
| B27 Plus | — | — |
Step 3: endothelial 2
day 5 ~ 6 (duration missing in database; assumed 1 day)
culture “adherent” human PSC in “EGM2”. For ECM, use “Collagen” at “N/A” “N/A”. Medium should contain factors shown in the following table.
| concentration | unit | |
|---|---|---|
| VEGF | — | — |
Ideally, “EC” should be obtained at the end of culture.