Self-assembling 3D vessel-on-chip model with hiPSC-derived astrocytes

DOI: 10.1016/j.stemcr.2024.05.006

Authors: Dennis M. Nahon; Marc Vila Cuenca; Francijna E. van den Hil; Michel Hu; Tessa de Korte; Jean-Philippe Frimat; Arn M.J.M. van den Maagdenberg; Christine L. Mummery; Valeria V. Orlova

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “EC” should be obtained at the end of culture.