Direct programming of human pluripotent stem cells into endothelial progenitors with SOX17 and FGF2

DOI: 10.1016/j.stemcr.2024.02.006

Authors: Michael W. Ream; Lauren N. Randolph; Yuqian Jiang; Yun Chang; Xiaoping Bao; Xiaojun Lance Lian

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. Medium should contain factors shown in the following table.

Step 2: endothelial 1

day 1 ~ 2

culture “adherent” human PSC in “E8” for “1” days. “PSC” should be “upregulated” for “SOX17”. For ECM, use “Laminin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “EP” should express “CD34”, “CDH5”, “PECAM1” at the end of culture.

Step 3: endothelial 2

day 2 ~ 33

culture “adherent” human EP in “EGM2” for “31” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “31” days.

Ideally, “EC” should express “CDH5”, “PECAM1” at the end of culture.