Single-cell transcriptomic analysis of corneal organoids during development

DOI: 10.1016/j.stemcr.2023.10.022

Authors: Aditi Swarup; Ragini Phansalkar; Maurizio Morri; Aditi Agarwal; Varun Subramaniam; BaoXiang Li; Albert Y. Wu

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”.

Ideally, “mesoderm” should express “TMEM88”, “ACTA2”, “DLK1” at the end of culture.

Step 3: endothelial 1

day 2 ~ 3 (duration missing in database; assumed 1 day)

culture “spheroid” human PSC in “unspecified medium”.

Ideally, “EC” should express “PECAM1” at the end of culture.

Step 4: endothelial 2

day 3 ~ 4 (duration missing in database; assumed 1 day)

culture “spheroid” human PSC in “unspecified medium”.

Ideally, “EC” should express “COL8A2”, “AQP1”, “CRYAB”, “VEGF” at the end of culture.