Generation of anti-GD2 CAR macrophages from human pluripotent stem cells for cancer immunotherapies

DOI: 10.1016/j.stemcr.2022.12.012

Authors: Jue Zhang; Sarah Webster; Bret Duffin; Matthew N. Bernstein; John Steill; Scott Swanson; Matthew H. Forsberg; Jennifer Bolin; Matthew E. Brown; Aditi Majumder; Christian M. Capitini; Ron Stewart; James A. Thomson; Igor I. Slukvin

Step 1: endothelial 1

day 0 ~ 6

culture “unspecified method” unknown species cell in “unspecified medium” for “6” days. Medium should be changed once in “6” days.

Ideally, “EC” should express “EFNB2” at the end of culture.