Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiation
DOI: 10.1016/j.stemcr.2022.11.003
Authors: Kris G. Alavattam; Katie A. Mitzelfelt; Giancarlo Bonora; Paul A. Fields; Xiulan Yang; Han Sheng Chiu; Lil Pabon; Alessandro Bertero; Nathan J. Palpant; William S. Noble; Charles E. Murry
Step 1: iPSC/ESC maintainance
day 0 ~ 1 (duration missing in database; assumed 1 day)
culture “unspecified method” human ESC in “unspecified medium”.
Ideally, “ESC” should be obtained at the end of culture.
Step 2: mesoderm 1
day 1 ~ 2 (duration missing in database; assumed 1 day)
culture “unspecified method” human ESC in “unspecified medium”.
Ideally, “MES” should be obtained at the end of culture.
Step 3: endothelial 1
day 2 ~ 3 (duration missing in database; assumed 1 day)
culture “unspecified method” human MES in “unspecified medium”. Medium should contain factors shown in the following table.
| concentration | unit | |
|---|---|---|
| BMP4 | — | — |
| FGF | — | — |
| VEGF | — | — |
Ideally, “EP” should express “MECOM” at the end of culture.
Step 4: endothelial 2
day 3 ~ 4 (duration missing in database; assumed 1 day)
culture “unspecified method” human EP in “unspecified medium”.
Ideally, “EC” should express “PECAM1”, “KLF6”, “MECOM”, “VEGFC”, “KDR”, “TFPI” at the end of culture.