Laminin 411 mediates endothelial specification via multiple signaling axes that converge on β-catenin

DOI: 10.1016/j.stemcr.2022.01.005

Authors: Mikayla L. Hall; Sophie Givens; Natasha Santosh; Michelina Iacovino; Michael Kyba; Brenda M. Ogle

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” unknown species cell in “unspecified medium”. For ECM, use “LN411 + FN” at “N/A” “N/A”.

Step 3: mesoderm 1

day 2 ~ 3 (duration missing in database; assumed 1 day)

culture “adherent” unknown species cell in “unspecified medium”. For ECM, use “LN411 + FN” at “N/A” “N/A”.

Step 4: endothelial 1

day 3 ~ 17

culture “adherent” human PSC in “unspecified medium” for “14” days. For ECM, use “LN411 + FN” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “14” days.

Ideally, “EC” should express “PECAM1” at the end of culture.