Transcriptomically Guided Mesendoderm Induction of Human Pluripotent Stem Cells Using a Systematically Defined Culture Scheme

DOI: 10.1016/j.stemcr.2019.11.001

Authors: Richard L. Carpenedo; Sarah Y. Kwon; R. Matthew Tanner; Julien Yockell-Lelièvre; Chandarong Choey; Carole Doré; Mirabelle Ho; Duncan J. Stewart; Theodore J. Perkins; William L. Stanford

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 2

culture “adherent” human PSC in “E6 medium” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “PS” should be obtained at the end of culture.

Step 3: endothelial 1

day 2 ~ 8

culture “unspecified method” human PSC in “unspecified medium” for “6” days. Medium should be changed once in “6” days.

Ideally, “EC” should be obtained at the end of culture.