Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency

DOI: 10.1016/j.stemcr.2019.10.009

Authors: Nicholas Bredenkamp; Jian Yang; James Clarke; Giuliano Giuseppe Stirparo; Ferdinand von Meyenn; Sabine Dietmann; Duncan Baker; Rosalind Drummond; Yongming Ren; Dongwei Li; Chuman Wu; Maria Rostovskaya; Sarah Eminli-Meissner; Austin Smith; Ge Guo

Step 1: iPSC/ESC maintainance

day 0 ~ 10

culture “adherent” human PSC in “N2B27” for “10” days. Medium should contain factors shown in the following table, and should be changed once in “10” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 10 ~ 16

culture “unspecified method” human PSC in “unspecified medium” for “6” days. Medium should contain factors shown in the following table, and should be changed once in “6” days.