Closing the Mitochondrial Permeability Transition Pore in hiPSC-Derived Endothelial Cells Induces Glycocalyx Formation and Functional Maturation

DOI: 10.1016/j.stemcr.2019.10.005

Authors: Gesa L. Tiemeier; Gangqi Wang; Sébastien J. Dumas; Wendy M.P.J. Sol; M. Cristina Avramut; Tobias Karakach; Valeria V. Orlova; Cathelijne W. van den Berg; Christine L. Mummery; Peter Carmeliet; Bernard M. van den Berg; Ton J. Rabelink

Step 1: iPSC/ESC maintainance

day 0 ~ 4

culture “adherent” human PSC in “E8” for “4” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “4” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 4 ~ 5 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Step 3: Primitive streak 2

day 5 ~ 6 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Step 4: mesoderm 1

day 6 ~ 9

culture “adherent” human PSC in “unspecified medium” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Step 5: mesoderm 2

day 9 ~ 10 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Step 6: endothelial 1

day 10 ~ 17

culture “adherent” human PSC in “unspecified medium” for “7” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “7” days.

Ideally, “EC” should be obtained at the end of culture.