Differentiation and Functional Comparison of Monocytes and Macrophages from hiPSCs with Peripheral Blood Derivatives

DOI: 10.1016/j.stemcr.2019.05.003

Authors: Xu Cao; Gopala K. Yakala; Francijna E. van den Hil; Amy Cochrane; Christine L. Mummery; Valeria V. Orlova

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Matrigel” at “0.075” “g/l”. Medium should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 3

culture “adherent” human PSC in “IF9S medium” for “2” days. For ECM, use “Matrigel” at “0.075” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “mesoderm” should be obtained at the end of culture.

Step 3: endothelial 1

day 3 ~ 6

culture “adherent” human PSC in “IF9S medium” for “3” days. For ECM, use “Matrigel” at “0.075” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “HE” should be obtained at the end of culture.

Step 4: endothelial 2

day 6 ~ 10

culture “adherent” unknown species cell in “IF9S medium” for “4” days. For ECM, use “Matrigel” at “0.075” “g/l”. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “HPC” should be obtained at the end of culture.