A Human Pluripotent Stem Cell-Based Screen for Smooth Muscle Cell Differentiation and Maturation Identifies Inhibitors of Intimal Hyperplasia

DOI: 10.1016/j.stemcr.2019.04.013

Authors: Jue Zhang; Brian E. McIntosh; Bowen Wang; Matthew E. Brown; Mitchell D. Probasco; Sarah Webster; Bret Duffin; Ying Zhou; Lian-Wang Guo; William J. Burlingham; Craig Kent; Michael Ferris; James A. Thomson

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”.

Step 2: Primitive streak 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Matrigel” at “N/A” “N/A”.

Step 3: Primitive streak 2

day 2 ~ 3 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E6 medium”. For ECM, use “Matrigel” at “N/A” “N/A”.

Step 4: mesoderm 1

day 3 ~ 4 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “mesoderm” should express “MEOX1” at the end of culture.

Step 5: mesoderm 2

day 4 ~ 9

culture “adherent” human PSC in “unspecified medium” for “5” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “5” days.

Ideally, “mesoderm” should be obtained at the end of culture.