Automated Deep Learning-Based System to Identify Endothelial Cells Derived from Induced Pluripotent Stem Cells

DOI: 10.1016/j.stemcr.2018.04.007

Authors: Dai Kusumoto; Mark Lachmann; Takeshi Kunihiro; Shinsuke Yuasa; Yoshikazu Kishino; Mai Kimura; Toshiomi Katsuki; Shogo Itoh; Tomohisa Seki; Keiichi Fukuda

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” unknown species PSC in “mTeSR” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 2 ~ 5

culture “unspecified method” unknown species cell and unknown species PSC in “unspecified medium” for “3” days. Medium should be changed once in “3” days.

Ideally, “mesoderm” should be obtained at the end of culture.

Step 3: endothelial 1

day 5 ~ 7

culture “unspecified method” unknown species cell and unknown species mesoderm in “unspecified medium” for “2” days. Medium should be changed once in “2” days.

Ideally, “EC” should express “PECAM1” at the end of culture.