Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture

DOI: 10.1016/j.stemcr.2018.03.017

Authors: Ruth Olmer; Lena Engels; Abdulai Usman; Sandra Menke; Muhammad Nasir Hayat Malik; Frank Pessler; Gudrun Göhring; Dorothee Bornhorst; Svenja Bolten; Salim Abdelilah-Seyfried; Thomas Scheper; Henning Kempf; Robert Zweigerdt; Ulrich Martin

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “spheroid” human PSC in “unspecified medium”.

Step 2: mesoderm 1

day 1 ~ 3

culture “spheroid” human PSC in “N2B27 medium” for “2” days. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 3: endothelial 1

day 3 ~ 4

culture “spheroid” human PSC in “StemPro-34 medium” for “1” days. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “EC” should express “KDR”, “PECAM1”, “CDH5”, “POU5F1”, “NANOG”, “CD34”, “NOS3”, “VWF”, “DLL4”, “NRP1”, “NOTCH1”, “NRP2”, “EPHB4” at the end of culture.