Inflammatory Responses and Barrier Function of Endothelial Cells Derived from Human Induced Pluripotent Stem Cells

DOI: 10.1016/j.stemcr.2018.03.012

Authors: Oleh V. Halaidych; Christian Freund; Francijna van den Hil; Daniela C.F. Salvatori; Mara Riminucci; Christine L. Mummery; Valeria V. Orlova

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “EC” should express “VEC”, “PECAM1”, “CD34”, “KDR”, “CXCR4”, “VEGFR3”, “CD73”, “CD105”, “KDR”, “SOX17”, “NR2F2”, “APLNR”, “ZO1”, “VWF” at the end of culture.

Step 3: endothelial 2

day 2 ~ 3 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “EC” should express “VEC”, “PECAM1”, “CD34”, “KDR”, “CXCR4”, “VEGFR3”, “CD73”, “CD105”, “KDR”, “SOX17”, “NR2F2”, “APLNR”, “ZO1”, “VWF” at the end of culture.