MEIS1 Regulates Hemogenic Endothelial Generation, Megakaryopoiesis, and Thrombopoiesis in Human Pluripotent Stem Cells by Targeting TAL1 and FLI1

DOI: 10.1016/j.stemcr.2017.12.017

Authors: Hongtao Wang; Cuicui Liu; Xin Liu; Mengge Wang; Dan Wu; Jie Gao; Pei Su; Tatsutoshi Nakahata; Wen Zhou; Yuanfu Xu; Lihong Shi; Feng Ma; Jiaxi Zhou

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “mTeSR” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 3

culture “adherent” human PSC in “mTeSR” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 3: mesoderm 1

day 3 ~ 5

culture “adherent” unknown species cell in “mTeSR” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 4: mesoderm 2

day 5 ~ 8

culture “adherent” human mesoderm in “mTeSR” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “HEP” should express “PECAM1”, “CD34” at the end of culture.