Transcriptionally and Functionally Distinct Mesenchymal Subpopulations Are Generated from Human Pluripotent Stem Cells

DOI: 10.1016/j.stemcr.2017.12.005

Authors: Chee Jia Chin; Suwen Li; Mirko Corselli; David Casero; Yuhua Zhu; Chong Bin He; Reef Hardy; Bruno Péault; Gay M. Crooks

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “unspecified medium”.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”.

Ideally, “mesoderm” should express “CD326”, “CD56” at the end of culture.

Step 3: mesoderm 2

day 2 ~ 16

culture “adherent” human mesoderm in “MesenCult” for “14” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “14” days.

Ideally, “EC” should express “PECAM1”, “PTPRC” at the end of culture. Ideally, “mesoderm” should express “THY1”, “CD105”, “CD44”, “PDGFRB”, “MCAM”, “CD73”, “CD73”, “CD140a” at the end of culture.