A Genome-wide Analysis of Human Pluripotent Stem Cell-Derived Endothelial Cells in 2D or 3D Culture

DOI: 10.1016/j.stemcr.2017.02.014

Authors: Jue Zhang; Michael P. Schwartz; Zhonggang Hou; Yongsheng Bai; Hamisha Ardalani; Scott Swanson; John Steill; Victor Ruotti; Angela Elwell; Bao Kim Nguyen; Jennifer Bolin; Ron Stewart; James A. Thomson; William L. Murphy

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “vitronectin” at “N/A” “N/A”.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “vitronectin” at “0.01” “g/m2”. Medium should contain factors shown in the following table.

Step 3: mesoderm 2

day 2 ~ 5

culture “adherent” human PSC in “E7 medium” for “3” days. For ECM, use “vitronectin” at “0.01” “g/m2”. Medium should be changed once in “3” days.

Step 4: endothelial 1

day 5 ~ 10

culture “adherent” human PSC in “E8” for “5” days. For ECM, use “vitronectin” at “0.01” “g/m2”. Medium should be changed once in “5” days.

Ideally, “EC” should be obtained at the end of culture.