Wnt/β-Catenin Stimulation and Laminins Support Cardiovascular Cell Progenitor Expansion from Human Fetal Cardiac Mesenchymal Stromal Cells

DOI: 10.1016/j.stemcr.2016.02.014

Authors: Agneta Månsson-Broberg; Sergey Rodin; Ivana Bulatovic; Cristián Ibarra; Marie Löfling; Rami Genead; Eva Wärdell; Ulrika Felldin; Carl Granath; Evren Alici; Katarina Le Blanc; C.I. Edvard Smith; Alena Salašová; Magnus Westgren; Erik Sundström; Per Uhlén; Ernest Arenas; Christer Sylvén; Karl Tryggvason; Matthias Corbascio; Oscar E. Simonson; Cecilia Österholm; Karl-Henrik Grinnemo

Step 1: endothelial 1

day 0 ~ 21

culture “adherent” human MSC in “EGM2” for “21” days. For ECM, use “LN-521/411” at “0.05” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “21” days.

Ideally, “EC” should express “PECAM1” at the end of culture.