CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells

DOI: 10.1016/j.stemcr.2015.11.006

Authors: Rhys J.P. Skelton; Bevin Brady; Suhail Khoja; Debashis Sahoo; James Engel; Deevina Arasaratnam; Kholoud K. Saleh; Oscar J. Abilez; Peng Zhao; Edouard G. Stanley; Andrew G. Elefanty; Murray Kwon; David A. Elliott; Reza Ardehali

Step 1: mesoderm 1

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”.

Ideally, “mesoderm” should express “MIXL1”, “PDGFRA”, “HAND1”, “MESP1”, “EOMES”, “CD13”, “ROR2” at the end of culture.

Step 2: endothelial 1

day 1 ~ 12

culture “unspecified method” human mesoderm in “unspecified medium” for “11” days. Medium should contain factors shown in the following table, and should be changed once in “11” days.

Ideally, “EC” should express “CDH5”, “TAL1”, “TEK”, “KDR”, “VWF”, “PECAM1”, “CD34” at the end of culture.