Induced Pluripotent Stem Cells to Model Human Fibrodysplasia Ossificans Progressiva

DOI: 10.1016/j.stemcr.2015.10.020

Authors: Jie Cai; Valeria V. Orlova; Xiujuan Cai; Elisabeth M.W. Eekhoff; Keqin Zhang; Duanqing Pei; Guangjin Pan; Christine L. Mummery; Peter ten Dijke

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “mTeSR”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: Primitive streak 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”. Medium should contain factors shown in the following table.

Ideally, “PS” should be obtained at the end of culture.

Step 3: mesoderm 1

day 2 ~ 3 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”. Medium should contain factors shown in the following table.

Ideally, “mesoderm” should be obtained at the end of culture.

Step 4: endothelial 1

day 3 ~ 13

culture “unspecified method” unknown species cell in “unspecified medium” for “10” days. Medium should be changed once in “10” days.

Ideally, “EC” should be obtained at the end of culture. Ideally, “MC” should be obtained at the end of culture.

Step 5: endothelial 2

day 13 ~ 14 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “EGM2”. Medium should contain factors shown in the following table.

Ideally, “EC” should be obtained at the end of culture. Ideally, “MC” should be obtained at the end of culture.