Repair of Ischemic Injury by Pluripotent Stem Cell Based Cell Therapy without Teratoma through Selective Photosensitivity

DOI: 10.1016/j.stemcr.2015.10.004

Authors: Seung-Ju Cho; So-Yeon Kim; Ho-Chang Jeong; Hyeonsik Cheong; Doseok Kim; Soon-Jung Park; Jong-Jin Choi; Hyongbum Kim; Hyung-Min Chung; Sung-Hwan Moon; Hyuk-Jin Cha

Step 1: Primitive streak 1

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “spheroid” mouse ESC in “unspecified medium”.

Step 2: Primitive streak 2

day 1 ~ 5

culture “adherent” mouse ESC in “unspecified medium” for “4” days. Medium should be changed once in “4” days.

Ideally, “dummy” should express “Fgf5”, “TBXT”, “SOX17” at the end of culture.

Step 3: endothelial 1

day 5 ~ 6 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell and mouse ESC in “unspecified medium”.

Ideally, “EC” should express “Vwf” at the end of culture.