Functional Vascular Endothelium Derived from Human Induced Pluripotent Stem Cells
DOI: 10.1016/j.stemcr.2013.06.007
Authors: William J. Adams; Yuzhi Zhang; Jennifer Cloutier; Pranati Kuchimanchi; Gail Newton; Seema Sehrawat; William C. Aird; Tanya N. Mayadas; Francis W. Luscinskas; Guillermo García-Cardeña
Step 1: iPSC/ESC maintainance
day 0 ~ 1 (duration missing in database; assumed 1 day)
culture “unspecified method” human PSC in “unspecified medium”.
Step 2: endothelial 1
day 1 ~ 11
culture “spheroid” human PSC in “unspecified medium” for “10” days. Medium should contain factors shown in the following table, and should be changed once in “10” days.
| concentration | unit | |
|---|---|---|
| FBS | — | — |
Ideally, “EC” should express “CDH5”, “KDR”, “PECAM1” at the end of culture.