Human vascularized macrophage-islet organoids to model immune-mediated pancreatic β cell pyroptosis upon viral infection

DOI: 10.1016/j.stem.2024.08.007

Authors: Liuliu Yang; Yuling Han; Tuo Zhang; Xue Dong; Jian Ge; Aadita Roy; Jiajun Zhu; Tiankun Lu; J. Jeya Vandana; Neranjan de Silva; Catherine C. Robertson; Jenny Z. Xiang; Chendong Pan; Yanjie Sun; Jianwen Que; Todd Evans; Chengyang Liu; Wei Wang; Ali Naji; Stephen C.J. Parker; Robert E. Schwartz; Shuibing Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “StemFlex medium”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “ESC” should be obtained at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” human ESC in “unspecified medium”. “ESC” should be “upregulated” for “ETV2”.

Ideally, “EC” should express “PECAM1” at the end of culture.