KSCBi005-A-10(hiPSC-HIF1αKO), a HIF1α knockout human induced pluripotent stem cell line, for demonstrating the role of cellular response to hypoxia

DOI: 10.1016/j.scr.2024.103415

Authors: Hyeong-jun Han; Jung-Hyun Kim

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. “PSC” should be “downregulated” for “HIF-1α”. For ECM, use “vitronectin” at “N/A” “N/A”.

Ideally, “PSC” should express “POU5F1”, “SSEA4”, “TRA-1-60”, “TRA-1-81” at the end of culture.