Generation of human induced pluripotent stem cell line EURACi015-A from a patient affected by dilated cardiomyopathy carrying the Lamin A/C p.Glu161Lys mutation

DOI: 10.1016/j.scr.2023.103172

Authors: Giada Cattelan; Laura Sophie Frommelt; Chiara Volani; Andrea Colliva; Giulio Ciucci; Alessia Paldino; Matteo Dal Ferro; Marina Di Segni; Rosamaria Silipigni; Peter P. Pramstaller; Marzia De Bortoli; Serena Zacchigna; Alessandra Rossini

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “StemMACS iPS-Brew XF”. “PSC” should be “unspecified regulation” for “LMNA”. For ECM, use “Matrigel” at “0.083” “g/l”. Medium should contain factors shown in the following table.

Ideally, “PSC” should express “POU5F1”, “SOX2”, “NANOG”, “TRA-1-60”, “SSEA4” at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” unknown species cell in “unspecified medium”.

Ideally, “EC” should express “PECAM1” at the end of culture.