Integration-free reprogramming of human umbilical arterial endothelial cells into induced pluripotent stem cells IHSTMi001-A

DOI: 10.1016/j.scr.2018.08.007

Authors: Huilin Li; Haiyun Pei; Jiqin Nie; Mingyi Qu; Zeng Fan; Yali Jia; Lijuan He; Xue Nan; Wen Yue; Xuetao Pei

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “E8”. For ECM, use “Geltrex” at “N/A” “N/A”.

Ideally, “PSC” should express “POU5F1”, “SOX2”, “NANOG”, “SSEA-4”, “TRA-1-60” at the end of culture.