Microgravity simulation activates Cdc42 via Rap1GDS1 to promote vascular branch morphogenesis during vasculogenesis

DOI: 10.1016/j.scr.2017.11.002

Authors: Shouli Wang; Zhao Yin; Bei Zhao; Yanmei Qi; Jie Liu; Saum A. Rahimi; Leonard Y. Lee; Shaohua Li

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” mouse ESC in “unspecified medium”.

Step 2: mesoderm 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “adherent” mouse ESC in “unspecified medium”. For ECM, use “FN1” at “N/A” “N/A”.

Ideally, “mesoderm” should express “TBXT”, “Eomesodermin” at the end of culture.

Step 3: endothelial 1

day 2 ~ 4

culture “adherent” mouse ESC in “unspecified medium” for “2” days. For ECM, use “FN1” at “N/A” “N/A”. Medium should be changed once in “2” days.

Ideally, “EC” should express “PECAM1” at the end of culture.

Step 4: endothelial 2

day 4 ~ 5

culture “adherent” mouse ESC in “unspecified medium” for “1” days. For ECM, use “FN1” at “N/A” “N/A”. Medium should be changed once in “1” days.

Ideally, “EC” should express “KDR”, “CDH5” at the end of culture.