ID3 contributes to the acquisition of molecular stem cell-like signature in microvascular endothelial cells: Its implication for understanding microvascular diseases

DOI: 10.1016/j.mvr.2015.01.006

Authors: Jayanta K. Das; Norbert F. Voelkel; Quentin Felty

Step 1: endothelial 1

day 0 ~ 4

culture “spheroid” human EC in “DMEM/F12” for “4” days. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “EC” should be obtained at the end of culture.

Step 2: endothelial 2

day 4 ~ 19

culture “spheroid” human EC and human Pericyte/SMC in “DMEM/F12” for “15” days. “EC” should be “upregulated” for “ID3”. For ECM, use “collagen; Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “15” days.

Ideally, “EC” should express “CD34” at the end of culture. Ideally, “Pericyte/SMC” should express “ACTA2” at the end of culture.