The method to generate pulsatile circulatory fluid flow using microfluidics

DOI: 10.1016/j.mex.2021.101269

Authors: Jingjing Li; Osmond Lao; Robert E. Nordon

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “E8”. For ECM, use “Geltrex” at “1” “%”.

Ideally, “ESC” should be obtained at the end of culture.