Chronic nicotine impairs the angiogenic capacity of human induced pluripotent stem cell-derived endothelial cells in a murine model of peripheral arterial disease

DOI: 10.1016/j.jvssci.2023.100115

Authors: Alex H.P. Chan; Caroline Hu; Gladys C.F. Chiang; Chisomaga Ekweume; Ngan F. Huang

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “E8”.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 5

culture “spheroid” human PSC in “unspecified medium” for “4” days. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “mesoderm” should be obtained at the end of culture.

Step 3: endothelial 1

day 5 ~ 15

culture “adherent” human mesoderm in “unspecified medium” for “10” days. For ECM, use “Gelatin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “10” days.

Ideally, “EC” should be obtained at the end of culture.