Liver specification of human iPSC-derived endothelial cells transplanted into mouse liver

DOI: 10.1016/j.jhepr.2024.101023

Authors: Kiryu K. Yap; Jan Schröder; Yi-Wen Gerrand; Aleksandar Dobric; Anne M. Kong; Adrian M. Fox; Brett Knowles; Simon W. Banting; Andrew G. Elefanty; Eduoard G. Stanley; George C. Yeoh; Glen P. Lockwood; Victoria C. Cogger; Wayne A. Morrison; Jose M. Polo; Geraldine M. Mitchell

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “E8” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 4

culture “adherent” human PSC in “DMEM/F12” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Step 3: endothelial 1

day 4 ~ 7

culture “adherent” human PSC in “StemPro” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “EC” should be obtained at the end of culture.

Step 4: endothelial 2

day 7 ~ 8 (duration missing in database; assumed 1 day)

culture “adherent” human EC in “EGM-2-MV”. For ECM, use “FN1” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “EC” should express “PECAM1”, “VWF”, “VEGFR”, “CDH5” at the end of culture.