Targeted proteomics for absolute quantification of glucose transporter 1 in mammalian brain cells using liquid chromatography-mass spectrometry

DOI: 10.1016/j.jcoa.2024.100198

Authors: Yash Mehta; Dhavalkumar Patel; Iqra Pervaiz; Ulrich Bickel; Abraham Jacob Al-Ahmad

Step 1: iPSC/ESC maintainance

day 0 ~ 4

culture “adherent” human PSC in “E8” for “4” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should be changed once in “4” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: endothelial 1

day 4 ~ 10

culture “adherent” human PSC in “DMEM/F12” for “6” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “6” days.

Step 3: endothelial 2

day 10 ~ 12

culture “adherent” human PSC in “ECSFM” for “2” days. For ECM, use “Collagen” at “0.8” “g/m2”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “EC” should be obtained at the end of culture.