Patient-Induced Pluripotent Stem Cell–Derived Hepatostellate Organoids Establish a Basis for Liver Pathologies in Telomeropathies

DOI: 10.1016/j.jcmgh.2023.06.003

Authors: Young-Jun Choi; Melissa S. Kim; Joshua H. Rhoades; Nicolette M. Johnson; Corbett T. Berry; Sarah Root; Qijun Chen; Yuhua Tian; Rafael J. Fernandez; Zvi Cramer; Stephanie Adams-Tzivelekidis; Ning Li; F. Brad Johnson; Christopher J. Lengner

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human PSC in “StemMACS iPSC-Brew XF medium” for “1” days. “PSC” should be “unspecified regulation” for “DKC1”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “PSC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 5

culture “unspecified method” human PSC in “DMEM low glucose” for “4” days. Medium should contain factors shown in the following table, and should be changed once in “4” days.

Ideally, “mesoderm” should be obtained at the end of culture.