Effect of Human Donor Cell Source on Differentiation and Function of Cardiac Induced Pluripotent Stem Cells

DOI: 10.1016/j.jacc.2014.04.056

Authors: Veronica Sanchez-Freire; Andrew S. Lee; Shijun Hu; Oscar J. Abilez; Ping Liang; Feng Lan; Bruno C. Huber; Sang-Ging Ong; Wan Xing Hong; Mei Huang; Joseph C. Wu

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “unspecified medium”. For ECM, use “Matrigel” at “N/A” “N/A”.

Ideally, “PSC” should express “TRA-1-60”, “POU5F1”, “NANOG” at the end of culture.

Step 2: endothelial 1

day 1 ~ 2 (duration missing in database; assumed 1 day)

culture “unspecified method” human PSC in “unspecified medium”.

Ideally, “EC” should express “PECAM1”, “VWF” at the end of culture.