SPI1-KLF1/LYL1 axis regulates lineage commitment during endothelial-to-hematopoietic transition from human pluripotent stem cells

DOI: 10.1016/j.isci.2024.110409

Authors: Kengyuan Qu; Shaokang Mo; Junfeng Huang; Shan Liu; Shuo Zhang; Jun Shen; Kuangyu Yen

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human ESC in “E8” for “1” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Step 2: Primitive streak 1

day 1 ~ 3

culture “adherent” human ESC in “STEMdiff APEL 2 Medium” for “2” days. For ECM, use “vitronectin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 3: endothelial 1

day 3 ~ 4

culture “adherent” human ESC in “STEMdiff APEL 2 Medium” for “1” days. For ECM, use “vitronectin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Step 4: endothelial 2

day 4 ~ 7

culture “adherent” human ESC in “STEMdiff APEL 2 Medium” for “3” days. For ECM, use “vitronectin” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.