SETD7 promotes lateral plate mesoderm formation by modulating the Wnt/β-catenin signaling pathway

DOI: 10.1016/j.isci.2023.106917

Authors: Ding Wang; Yapu Li; Changlu Xu; Hongtao Wang; Xin Huang; Xu Jin; Sirui Ren; Jie Gao; Jingyuan Tong; Jinhua Liu; Jiaxi Zhou; Lihong Shi

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human ESC in “mTeSR”. “ESC” should be “downregulated” for “SETD7”. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table.

Ideally, “ESC” should be obtained at the end of culture.

Step 2: mesoderm 1

day 1 ~ 3

culture “adherent” human ESC in “mTeSR” for “2” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “mesoderm” should express “APLNR”, “SETD7” at the end of culture.

Step 3: endothelial 1

day 3 ~ 6

culture “adherent” human mesoderm in “mTeSR” for “3” days. For ECM, use “Matrigel” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “3” days.

Ideally, “EPC” should express “PECAM1”, “CD34” at the end of culture.