SOX18-enforced expression diverts hemogenic endothelium-derived progenitors from T towards NK lymphoid pathways

DOI: 10.1016/j.isci.2023.106621

Authors: Ho Sun Jung; Kran Suknuntha; Yun Hee Kim; Peng Liu; Samuel T. Dettle; Divine Mensah Sedzro; Portia R. Smith; James A. Thomson; Irene M. Ong; Igor I. Slukvin

Step 1: iPSC/ESC maintainance

day 0 ~ 1

culture “adherent” human cell in “E8” for “1” days. For ECM, use “Collagen IV” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Step 2: Primitive streak 1

day 1 ~ 2

culture “adherent” unknown species cell in “unspecified medium” for “1” days. For ECM, use “Collagen IV” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Step 3: mesoderm 1

day 2 ~ 4

culture “adherent” human cell in “unspecified medium” for “2” days. “cell” should be “upregulated” for “SOX18”. For ECM, use “Collagen IV” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Step 4: endothelial 1

day 4 ~ 5

culture “adherent” human cell in “unspecified medium” for “1” days. “cell” should be “upregulated” for “SOX18”. For ECM, use “Collagen IV” at “N/A” “N/A”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “EC” should be obtained at the end of culture. Ideally, “HE” should be obtained at the end of culture.