Reconstituted basement membrane enables airway epithelium modeling and nanoparticle toxicity testing

DOI: 10.1016/j.ijbiomac.2022.02.018

Authors: Elrade Rofaani; Boxin Huang; Feng Liang; Juan Peng; Yong Chen

Step 1: iPSC/ESC maintainance

day 0 ~ 2

culture “adherent” human PSC in “E8 Flex medium” for “2” days. For ECM, use “vitronectin” at “1” “%”. Medium should be changed once in “2” days.

Ideally, “PSC” should be obtained at the end of culture.