An unexpected role of IL10 in mesoderm induction and differentiation from pluripotent stem cells: Implications in zebrafish angiogenic sprouting, vascular organoid development, and therapeutic angiogenesis

DOI: 10.1016/j.ejcb.2024.151465

Authors: Kaiyuan Niu; Chengxin Zhang; Chenxin Liu; Wei Wu; Yi Yan; Ancheng Zheng; Silin Liu; Zhenning Shi; Mei Yang; Wen Wang; Qingzhong Xiao

Step 1: iPSC/ESC maintainance

day 0 ~ 1 (duration missing in database; assumed 1 day)

culture “adherent” human PSC in “mTeSR”. For ECM, use “Matrigel” at “10” “%”. Medium should contain factors shown in the following table.

Ideally, “PSC” should express “POU5F1”, “NANOG”, “SOX2” at the end of culture.

Step 2: mesoderm 1

day 1 ~ 2

culture “adherent” human PSC in “N2B27 medium” for “1” days. For ECM, use “Matrigel” at “10” “%”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Ideally, “mesoderm” should express “TBXT” at the end of culture.

Step 3: endothelial 1

day 2 ~ 3

culture “adherent” human mesoderm in “StemPro” for “1” days. For ECM, use “Matrigel” at “10” “%”. Medium should contain factors shown in the following table, and should be changed once in “1” days.

Step 4: endothelial 2

day 3 ~ 5

culture “adherent” human EC in “StemPro” for “2” days. For ECM, use “Matrigel” at “10” “%”. Medium should contain factors shown in the following table, and should be changed once in “2” days.

Ideally, “EC” should express “FLT1”, “CD31/PECAM1”, “CD144/VE-cadherin”, “NOS3”, “VWF” at the end of culture.